Identification of Dammarane-type Triterpenoid Saponins from the Root of Panax ginseng

The root of Panax ginseng, is a Korea traditional medicine, which is used in both raw and processed forms due to their different pharmacological activities. As part of a continued chemical investigation of ginseng, the focus of this research is on the isolation and identification of compounds from Panax ginseng root by open column chromatography, medium pressure liquid chromatography, semi-preparative-high performance liquid chromatography, Fast atom bombardment mass spectrometric, and nuclear magnetic resonance. Dammarane-type triterpenoid saponins were isolated from Panax ginseng root by open column chromatography, medium pressure liquid chromatography, and semi-preparative-high performance liquid chromatography. Their structures were identified as protopanaxadiol ginsenosides [gypenoside-V (1), ginsenosides-Rb1 (2), -Rb2 (3), -Rb3 (4), -Rc (5), and -Rd (6)], protopanaxatriol ginsenosides [20(S)-notoginsenoside-R2 (7), notoginsenoside-Rt (8), 20(S)-O-glucoginsenoside-Rf (9), 6-O-[alpha-L-rhamnopyranosyl(1-->2)-beta-D-glucopyranosyl]-20-O-beta-D-glucopyranosyl-3beta,12beta, 20(S)-dihydroxy-dammar-25-en-24-one (10), majoroside-F6 (11), pseudoginsenoside-Rt3 (12), ginsenosides-Re (13), -Re5 (14), -Rf (15), -Rg1 (16), -Rg2 (17), and -Rh1 (18), and vinaginsenoside-R15 (19)], and oleanene ginsenosides [calenduloside-B (20) and ginsenoside-Ro (21)] through the interpretation of spectroscopic analysis. The configuration of the sugar linkages in each saponin was established on the basic of chemical and spectroscopic data. Among them, compounds 1, 8, 10, 11, 12, 19, and 20 were isolated for the first time from P. ginseng root.

The Effects of Programmed Dietary System of a Mixture of Natural Supplements on the Composition, Serum Lipids and the Leptin Levels / 대한지역사회영양학회지

This study was to investigate the effects of a silk peptide, and prune and raspberry mixture on the body fat, serum lipid composition and fat distribution in Korean women during the time they received these supplements. During the 4 month period of this research (June to October, 2002), 75 women (average age 22.9 yr) were selected as subjects. Their nutrient intake was investigated by use of a questionnaire and the 24-hour recall method. Antropometric assessments of the subjects were investigated by using the SBIA Method (Segmental Bioimpedance Assay, Inbody 3.0). The results were as follows: their mean body weight was 59.3 kg, their mean body height was 161.7 cm and their mean Basal Metabolic Indices (BMI) was 22.7. The status of their dietary fiber intake significantly increased (p<0.05) after use of the supplements. Their body fat significantly decreased following the 3rd stage of supplementation (p<0.05), and their percent of body fat signiflcantly decreased after supplementation (p<0.05). Their triceps and suprailiac circumference significantly decreased following the 3rd stage of supplementation (p<0.05). Their waist circumferences and waist-hip ratios (WHR) significantly decreased after both the 2nd and 3rd stage of supplementation (p<0.05). Their total cholesterol significantly decreased following the 3rd stage of supplementation, as compared to their baseline results and those obtained following the 1st stage of supplementation (p<0.05). Their LDL-cholesterol and LDL/HDL ratios significantly decreased following the 3rd stage of supplementation, as compared to their baseline results and those obtained following the 1st stage of supplementation (p<0.05). Their 4-HNE and total antioxidant statuses significantly decreased following the 2nd and 3rd stage of supplementation, as compared to their baseline results and those obtained following the 1st stage of supplementation (p<0.05). Their 2nd stage supplementation results were significantly negatively correlated with their triceps measurements and WHR (p<0.05). Their 3rd stage supplementation results were significantly negatively correlated with their anterior thigh measurements (p<0.05). The above results show that prune, raspberry, silk peptide and dietary fiber supplementation to a routine diet improves body fat distribution, total cholesterol, and triglyceride levels. The decrease of abdominal fat and WHR were especially noteworthy. This implies a decrease of risk factors.